【Abstract】 Objective To investigate the association between βtrophin and nonalcoholic fatty liver disease (NAFLD). Methods 100 patients with NAFLD (test group) and 50 patients with normal physical examination (control group) treated in our hospital from January 2016 to August 2016 were selected as study subjects. Retrospective analysis was used to analyze the clinical value and followup data of all the investigators. All patients underwent abdominal Bultrasound examination, and detection of serum βtrophin, liver and kidney function related biochemical indicators. According to Bultrasound results, 100 patients with NAFLD were classified as mild, moderate and severe, compare the differences in the above data among different investigators and analyze the correlation between serum βtrophin and other indicators. Investigate the relationship between serum βtrophin expression and NAFLD. Results There was no difference in levels of Cr, BUN, and UA between the control group and NAFLD patients (P＞005). However, the levels of serum βtrophin, AST, and ALT in patients with NAFLD were significantly higher than those in the control group. And as the condition worsens, the above indicators are higher (P＜005). There was no difference in the FPG level between the control group and NAFLD patients (P＞005). However, the levels of TG, LDLc, FINS, HbAlc and HOMAIR in NAFLD patients were significantly higher than those in the control group, and HDLC was lower than that in the control group, with the severity of the disease, the above indicators increased and decreased greater, and the differences were statistically significant (P＜005). Spearman rank correlation analysis showed that serum βtrophin was positively correlated with AST, ALT, TG, LDLc, FINS, HbAlc, and HOMAIR, but negatively correlated with HDLC (P＜005). Conclusion Serum level of βtrophin in NAFLD patients are significantly higher than those in normal subjects, and are positively correlated with AST, ALT, TG, LDLc, FINS, HbAlc, and HOMAIR. It is believed that βtrophin may have a certain biochemical effect on insulin resistance and lipid metabolism, resulting in the occurrence and progression of NAFLD.