【Abstract】 Objective To investigate and analyze the prevalence of Helicobacter pylori antibiotic resistance.Methods The antibiotic resistance of H. pylori strains isolated from 14882 gastric biopsies of patients inspected at Daping Hospital from January 2015 to December 2017 was assessed by agar dilution method，and statistical analysis was performed on the results of drug sensitivity. Results 3841 Helicobacter pylori strains were isolated. The positive rate of isolated culture was 2581%. The male positive rate was significantly higher than female positive rate (P＜005). The resistance rates to metronidazole，clarithromycin, levofloxacin and amoxicillin were 9409%,2630%, 3398% and 026%, respectively. No resistance to furazolidone or tetracycline was observed. When comparing with those in the past three years, the antibiotic resistance rates to metronidazole，clarithromycin and levofloxacin were increased from 8897%,2241% , 2172% to 9688%,2587% , 3683%(P＜005). Higher resistance to clarithromycin and levofloxacin were observed in female patients. As the growth of the age, we also noted a remarkable rise in metronidazole resistance and levofloxacin resistance. No difference of resistance to clarithromycin was observed among various age groups. Conclusion The prevalence of Hp resistance to clarithromycin, metronidazole and levofloxacin in Chongqing is high. Choice of therapy should be individualized based on a susceptibilitytest in this region, or bismuthcontaining quadruple therapies with a combination of alternative antibiotics (eg, amoxicillin, tetracycline, furazolidone or metronidazole) for empiric treatment should be recommended.