【Abstract】 Objective To study the recovery efficiency and quality control system for poliovirus Type I Sabin strain, purified by ion exchange. Methods Vero cells were seeded on the microcarrier, infected by poliovirus type I Sabin strains. After harvesting of virus, the systematic purification process was taken, including multistage filtration clarification, 100K ultrafiltration membrane and then gel medium. The purification efficiency of ion medium DEAE Sepharose FF chromatography was observed for 5 batches. The key quality control points including D antigen, protein content, bovine serum albumin content and DNA residue were monitored and evaluated for each times. Results After purified poliovirus Type I Sabin strain by DEAE Sepharose FF, there was no significant difference in D antigen, protein content, bovine serum albumin content, DNA residue and specific activity between each batch. Conclusion The purification method of DEAE Sepharose FF for poliovirus Type I Sabin strain was useful and stability.