Objective To study the effects of low frequency ultrasound on the drug release from inorganic hollow nanospheres and investigate the possibility of inorganic hollow nanospheres as the carrier of ultrasound targeted drug delivery system. Methods Using phytic acid as the modifier and calcium salt and selenite as reagent, inorganic hollow nanospheres were synthesized under hydrothermal condition. Then, bovine serum albumin as the model drug were loaded into the nanospheres and stimulated by low frequency ultrasound. The BSA release amount was measured under the low frequency ultrasound stimulus. Results Inorganic hollow nanospheres with good dispensability were successfully synthesized under hydrothermal condition. BSA release was facilated under ultrasound stimulus and BSA release rate increased with the ultrasound power. Conclusion This method is simple and effective to synthesize inorganic hollow nanospheres with good dispensability. Moreover, low frequency ultrasound stimulus is benefit to facilate drug release from inorganic hollow nanospheres. The inorganic hollow nanospheres could be a potent carrier of low frequency ultrasound targeted drug delivery system.