Objective To evaluate the construction and heart function changes in the mouse model of minimally invasive transverse aortic constriction (MITAC) with echocardiography and discuss the stability and effectiveness of this model for simulating the pathophysiology of heart failure. Methods The 8-10 weeks male C57BL/6 mice were randomly divided into Sham group and MITAC group. The aortic arch was isolated through modified suprasternal approach. The aortic arch was clamped with titanium clip in the MITAC group, but not in the Sham group. An echo system for small animals was used for measuring the blood flow velocity at aortic arch in the 1stweek after surgery, as well as left ventricular end-systolic internal dimension (LVIDs), left ventricular enddiastolic internal dimension (LVIDd) and left ventricular posterior wall enddiastolic thickness (LVPWd) in the 1st, 4thand 8thweek after surgery. Meanwhile, the ejection fraction and fractional shortening was calculated. Statistical analysis was performed for echo data in these two groups. Results Both groups enrolled 12 mice and the surgeries were successful for all the mice. Except for one mice (83%) in the MITAC group died after surgery, other mice were alive till the 8th week after surgery. Comparing MITAC group with Sham group, the blood flow velocity at aortic arch in the 1stweek after surgery increased significantly (P＜0.001). The ventricular wall thickness increased obviously in the 4th week after surgery (P＜0.01). The left ventricular internal dimensions were enlarged and systolic function decreased significantly in the 8thweek after surgery (P＜0.001). Conclusion As a noninvasive examination technique, echocardiography played an important role in the dynamic evaluation for MITAC mouse model and identification for stable and effective establishment of heart failure model.