【Abstract】 Objective To investigate the expression changes of intestinal fat absorption associated apoB48, apoAIV and MTP in highfat diet induced obesity rats. Methods 21dayold,weaning for 3 days healthy male SpragueDawley rats were randomized into normal control (n=20) and high fat diet group (n=40), fed with standard chow (290 kcal/100 g) and highfat how (430 kcal/100 g) for 24 weeks, respectively. Food and water were supplied ad libitum and rats were housed in independently ventilated animal cages on a 12:12h light: dark schedule and kept at 2025°C. Weight and body and tail lengths were measured weekly during the next 24 weeks feeding. Mean body weights, Lee’s indexes and abdominal fat were measured. Dietary and fetal fat were measured. Intestinal fat absorption ratio was calculated. Fasting glucose and lipids in the peripheral circulation were measured by enzymatic colorimetric method. Fasting serum insulin was measured by radioimmunoassay method to calcluded homeostatic model assessment (HOMA) value. Intestinal apoB48, apoAIV and MTP mRNA expression levels were determined by RTPCR and Realtime PCR. Intestinal MTP protein expression levels were determined by Western blot assay. ApoB48 levels in the small intestine homogenate were determined by ELISA. Results Rats with highfat diet induced obesity showed higher body weight, Lee’s index, abdominal fat and intestinal fat absorption ratio, along with higher FPG, TG, TC, fasting serum insulin and HOMA value(P＜005). RTPCR and Realtime PCR results showed that rats in the obese group had a higher levels of apoB, apoAIV and MTP mRNA than rats in control group (P＜001). Western blot results showed that MTP protein expression was enhanced in small intestine of obese group than control group (P＜001).Conclusion Highfat diet could induce overproduction of apoB48, MTP and apoAIV in the intestine and enhanced absorption of fat.