【Abstract】 Objective To investigate the inhibitory effect and mechanism of catalpol on the proliferation and apoptosis of ovarian cancer cells by regulating miR200 expression. Methods qPCR was used to detect the effect of different concentrations of catalpol on the cell viability of ovarian cancer cells. The effect of catalpol on the proliferation of ovarian cancer cells was analyzed. The effect of catalpol on the proliferation of ovarian cancer cells was detected by MTT assay. The effect of catalpol on the expression of caspase3 was detected by qPCR. Flow cytometry was used to detect the effect of catalpol on the apoptosis of ovarian cancer cells in a timedependent manner. Western blotting was used to detect the effect of catalpol on the expression of MMP2 in ovarian cancer cells. Results 100μg / ml catalpol had the best inhibitory effect on the cell activity of ovarian cancer cells. With the progress of time, catalpol directly inhibits the proliferation of ovarian cancer cells can interfere with the growth of ovarian cancer cells to a certain extent. Appropriate concentration of catalpol had a direct inhibitory effect on MMP2 protein in ovarian cancer cells. The transfection of miR200inhibitor can reverse the effect of catalpol on the proliferation of ovarian cancer cells to a certain extent.Conclusion Catalpol can inhibit the proliferation and promote the apoptosis of ovarian cancer cells by regulating the expression of miR200.