【Abstract】 Objective To investigate the effect of miR363 on the growth and invasion of nonsmall cell lung cancer cells by targeting HMGA2 and its mechanism. Methods qPCR was used to detect the expression of miR363 in nonsmall cell lung cancer and adjacent normal tissues. The relationship between the expression of miR363 and clinicopathological parameters of nonsmall cell lung cancer was analyzed. The proliferation of nonsmall cell lung cancer cells was detected by CCK8 cell proliferation assay. The effect of miR363 on cell cycle of nonsmall cell lung cancer was detected by flow cytometry. Scratch healing assay and Transwell invasion assay were used to detect the migration and invasion ability of miR363 cells after nonsmall cell lung cancer. Dual luciferase reporter assay detects the interaction of miR363 with HMGA2. Results The expression of miR363 in nonsmall cell lung cancer tissues was significantly higher than that in adjacent nonsmall cell lung cancer tissues and the highest expression level of miR363 in nonsmall cell lung cancer A549 cells. The inhibitory effect of miR363 on the proliferation of nonsmall cell lung cancer cells attenuated and promoted its apoptosis behavior. The inhibition of miR363 expression inhibited the migration and invasion ability of A549 cells accordingly. miR363 can specifically bind to the 3'UTR of HMGA2 and regulate the activity of HMGA2 expression. Conclusion miR363 plays an important role in the development and progression of nonsmall cell lung cancer and may affect the growth and invasion of nonsmall cell lung cancer cells by regulating the expression of HMGA2.