Objective To investigate the relationship between Highrisk HPV genotyping and cervical diseases, and provide scientific basis for prevention and treatment of cervical cancer. Methods Retrospective study was done to review 334 patients with different cervical lesions pathologically diagnosed and tested 15 subtypes(16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 66, 82) by PCR from July 2015 to March 2017. These patients were divided into the following groups, cervicitis (162 cases), cervical intraepithelium neoplasia grade I(CINI) (30 cases), grade II(CINII) (26 cases), grade III(CINIII) (50 cases) and cervical cancer (66 cases). The related data of the patients were analyzed by SPSS19.0. Results The total infection rate of hrHPV was 53.9%. The infection rates of CINII, III and cervical cancer were 92.3%, 92.0%, 97.0% respectively, higer than cervicitis 23.5% and CINI 26.7%, The infection rate of hrHPV was significantly different among varied cervical diseases (=1633, P＜0.01),and it gradually increased along with the increased disease grade(=148.2, P＜0.01). 180 patients infected with HPV. The single infection rates of CIN and cervical cancer were 746%, 781%,which were higher than that of cervicitis (368%). The single infection rate of hrHPV was significantly different among varied cervical diseases. As the severity of the disease increased, the HPV single infection rate increased significantly. The five most prevalence high risk types were 16(30.9%), 58 (15.4%), 52 (11.8%), 18 (8.1%), 33 (5.1%). The infection rate of HPV 16 were higher than that of HPV 58, 52, 18 and 33. The infection rate of hrHPV was significantly different among 16, 58, 52, 18 and 33. The positive rates in patients with 30-40, 41-50 and＞50 years old were 654%, 473%, 676%, which were higher than the positive rates 33.3% in＜30 years old. The infection rate of hrHPV was significantly different among varied Ages. Agespecific prevalence showed a “twopeak” pattern, with the 30-40 age group presenting the highest hrHPV infection rate (65.4 %), followed by a second peak(67.6%) for the＞50 years old group. Conclusion As the severity of the disease increased, the HPV infection rate increased significantly, the HPV single infection were the main ways in CIN and cervical cancer. The five most prevalence hrHPV subtypes were 16, 58, 52, 18, 33 in Nanchong, and＞30 years old. Therefore, we should pay great attention to more than 30 years old women who were infected hrHPV persistently, timely diagnosis and treatment for HPV infection could reduce the occurrence of highgrade CIN and cervical cancer.