Objective To understand the distribution and drug sensitivity of strains in pediatric inpatients, and provide evidence for diagnosis and treatment of clinical infectious diseases. Methods Bacterial culture data of paediatric inpatients from January 2016 to December 2017 were retrospectively analyzed, and the proportion of bacterial species and drug sensitivity were observed. Results 863 strains of bacteria were mainly detected in samples of sputum (86.6%) and blood (7.1%). Haemophilus influenzae, Streptococcus pneumoniae and Moraxelle catarrhalis were the major baceteria in sputum. While coagulase negative Staphylococcus and Enterobacteriaceae were main strains in blood. Gram positive cocci were mainly Streptococcus pneumoniae, which was resistant to tetracycline, erythromycin and compound neonammin, followed by Staphylococcus aureus with resistance to penicillin, erythromycin and clindamycin. Gramnegative bacteria were mainly Haemophilus influenzae and Moraxelle catarrhalis, which was resistant to ampicillin and SMZ TMP. And it was also included Klebsiella pneumoniae and Escherichia coli with resistance to penicillins and the third generation cephalosporins. Conclusion The major bacteria in paediatric inpatients are Haemophilus influenzae, Streptococcus pneumoniae and Moraxelle catarrhalis, which have different degree of resistance to commonly used antibiotics.Clinical should be based on the type of pathogen and sensitivity to antibiotics ,And the antibiotics should be selected correctly to control the production of multidrug resistant bacteria.