【Abstract】 Objective To lay the foundation for the assessment and cultivation of Master of Nursing Specialist, establish index system and evaluate the clinic competence of Master of Nursing Specialist based on standardized training mode. Methods The method of literature review and expert structured interview were used to built up the outline of assessment criteria. Delphi and Analytic Hierarchy Process (AHP) were used to inquiry experts about the criteria for two rounds. Results 36 valid questionnaires were included. The authority index of 40 experts inquired was 085. Determination Factor was 08. The familiarity was 09. Two rounds of questionnaire recovery rate were 95% and 947%, respectively. Coordination coefficient of level 1 and level 2 were 0415 and 0547, respectively (P < 005). Coordination degree of expert consultation was satisfactory. Eventually the assessment index system was formed, which covered seven level 1 index sort by weight, including professional skill, scientific research quality, theoretical knowledge, selfdevelopment ability, humanistic quality, teaching capacity, management ability, besides, thirtytwo level 2 index, fiftythree level 3 index. Conclusion In terms of the indicators used to assess the clinical competence of Master of Nursing Specialist based on standardized training mode, experts have reached unanimous opinion and maintained good coordination. Besides, the grading results are effective and researches are credible. It can provide the basis for the quality evaluation of the training scheme for the standardized training mode and nursing master degree.