【Abstract】 objective To compared therapeutic effect and serum IL6 and VB12 levels of the levamisole combining VB12 and levamisole in patients with recurrent oral ulcer and, explore the better treatment method for the recurrent oral ulcer. Methods 128 patients with recurrent oral ulcer were choosen as the research objects between September 2015 and September 2016 in our hospital division of the oral mucosa. The patients were randomly divided into levamisole combined VB12 groups and levamisole treatment group. The healing of oral ulcer, visual analog pain scale (visual analog scale for pain, VAS pain), serum IL6 and VB12 were observed. Results The whole test followup rate was 9375%. The number of losting to followup was 8 people. VAS score of levamisole combined VB12 treatment group was statistically significant lower than that of levamisole treatment group (P＜ 005). The oral ulcer healing rate of levamisole combined VB12 treatment group was statistically significant greater than that of levamisole treatment group (P＜ 005). The serum VB12 of levamisole combined VB12 combined group was statistically significant greater than that of levamisole treatment group (P＜ 005). The serum IL6 of levamisole combined VB12 combined group was statistically significant greater than that of levamisole treatment group (P＞005). Conclusion Levamisole combined VB12 has better effect of treatment for recurrent oral ulcer, the better treatment effect may be related with the higher level of serum IL6 and VB12. So clinical doctors can choose levamisole combined VB12 to cure the recurrent oral ulcer patients who are not well effent in traditional curation.