康复训练联合神经生长因子对脑出血大鼠肢体功能的影响
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重庆医药高等专科学校校级课题


Effects of rehabilitation training combined with nerve growth factor on limb function in rats with intracerebral hemorrhage
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    摘要:

    【摘要】 目的 观察康复训练联合神经生长因子(NGF)治疗脑出血大鼠后平衡木测评、转棒行走测评及网屏实验的变化情况。探讨康复训练联合神经生长因子治疗脑出血的作用机制。方法 将健康成年雄性SD大白鼠96只,随机分为对照组、康复训练组、NGF治疗组(腹腔内注射神经生长因子30ug/kg)和康复训练联合NGF治疗组,每组各24只。采用胶原酶诱导法建立大鼠脑出血动物模型。动物造模成功后分别按上述分组采用大鼠运动训练器材进行平衡、 旋转、抓握训练等治疗,并分别于第1、2周及第3周后采用平衡木行走测评、转棒行走测评、网屏实验评定运动功能,并观察不同治疗组对脑出血大鼠运动功能的影响。结果 康复训练联合NGF治疗组平衡木行走测试、转棒行走测评、网屏实验评定均高于康复训练组、NGF治疗组差异均有统计学意义(P均<005)。结论 康复训练和NGF单独治疗及联合应用治疗对脑出血大鼠神经功能恢复均有改善作用,但康复训练联合NGF治疗效果更佳且具有协同作用。

    Abstract:

    【Abstract】 Objective To observe the change of the balance beam test rotating rod walking test and screen experiment rehabilitation training combined with nerve growth factor in rats after cerebral hemorrhage and explore the mechanism of rehabilitation training combined with nerve growth factor in the treatment of cerebral hemorrhage. Method 96 Healthy adult male SD rats were randomly divided into control group, rehabilitation training group, NGF treatment group (intraperitoneal injection of nerve growth factor 30mg/kg) and rehabilitation training combined with NGF treatment group, 24 rats in each group. The animal model after the success of modeling respectively according to the above groups using rat exercise training equipment (for balancing, grasping, rotating and walking training) were treated respectively in the first weeks, the second weeks and the third weeks after the beam walking test, rotarod test, experimental evaluation of walking screen movement function, in order to study the effects of different treatment group of cerebral hemorrhage rats motor function.Results Balance beam walking test, rotarod test and experimental evaluation of walking screen of rehabilitation training combined NGF group were higher than that of rehabilitation group and NGF treatment group. Conclusion Rehabilitation training and NGF treatment alone and combined application of cerebral hemorrhage in rats have a positive role in the recovery of nerve function, but the combination of Rehabilitation training and NGF treatment has a synergistic effect.

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  • 在线发布日期: 2018-06-28
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