【Abstract】 Objective To study the subcellular localization of PEG10 gene in colorectal cancer cells, and explore the role of PEG10 in the invasion and growth of colorectal cancer. Methods Subcellular localization of PEG10 protein was detected by laser scanning confocal microscopy imaging. PEG10siRNA expression vector was constructed and stably transfected into SW620 cells. The expression of PEG10 gene was detected by Westernblot. The effect of PEG10 on the invasion and growth of SW620 cells was investigate with Transwell assay. Observing The tumor growth was observed when the SW620 cells after PEG10siRNA was injected in C57BL/6 mice. Results Laser scanning confocal microscopy imaging showed that PEG10 was mainly located in the cytoplasm. The interference plasmid PEG10 was successfully constructed. Transwell cell experiments indicated that siPEG10 inhibited colorectal cancer cell SW620 invasion ability. In vivo, siPEG10 SW620 cells were injected into subcutaneous tumor inhibited colorectal cancer cells growth compared with the control group. Conclusion PEG10 can promoted the invasion and growth of colorectal cancer cell lines in vitro and in vivo.