【Abstract】Objective To investigate the effect of curcumin combined with oxaliplatin on the biological behavior of leukemia cells. Methods Immunofluorescence was used to detect the fusion and efficiency of curcumin in HL-60 cells. The effects of different concentrations of curcumin and oxaliplatin on HL-60 cells were detected by MTT cell activity assay. Wright-Giemsa staining was used to observe the effect of curcumin and oxaliplatin on the morphological changes of HL-60 cells. The effects of curcumin and oxaliplatin on the migration of HL-60 cells were examined by scratch healing experiment. The effects of curcumin and oxaliplatin on the invasion of HL-60 cells were examined by transwell experiment.Results The fusion efficiency of liposomal curcumin in HL-60 cells was better. 10μM curcumin and 50 or 100μM oxaliplatin had the best effect on HL-60 cells. Wright-Giemsa staining showed that the effect of curcumin and oxaliplatin on the morphology and function of HL-60 cells was obvious. The proliferation, migration and invasion of HL-60 cells were significantly decreased after the action of curcumin and oxaliplatin on HL-60 cells, and the combined effect was better than separate medication.Conclusion Curcumin and oxaliplatin can effectively inhibit the proliferation, migration and invasion of leukemia HL60 cell line.