【Abstract】Objective To investigate the trend of antibacterial resistance and clinical distribution characteristics of Klebsiella pneumoniae from 2013 to 2016 and provide guidance for clinical rational use of antibacterial agents. Methods The reference of Klebsiella pneumoniae isolated from clinical specimens was analyzed retrospectively by WHONET 5.5 and SPSS 13.0 software from 2013 to 2016. Results Respiratory tract secretion and urine were the most common specimen source, accounting for 38.7% and 21.9% respectively. 9.8% of Klebsiella pneumoniae were isolated from department of hepatobiliary surgery, ranking first among all the departments. Klebsiella pneumoniae strains were highly resistant to ampicillin, and the resistance rate reached above 99%. The resistance rates of these strans to Carbapenems, cephamicins, piperacillintazobactam, cefepime, cefoperazonesulbactam, tobramycin and amikacin increased significantly (P＜0.01). The resistance rates of these strans to Nitrofurans and sulfonamides decreased significantly (P＜0.01). Above 99% of these strains were susceptible to tigecycline. The detection rates of muti-drug resistant and Carbapenemresistant Klebsiella pneumonia increased significantly (P＜0.01). The detection rate of extensivelydrug resistant Klebsiella pneumonia firstly decreased then increased significantly (P＜0.01). Conclusion The detection rates of mutidrug resistant, extensivelydrug resistant and carbapenemresistant Klebsiella pneumonia appeared increasing trend. Clinical workers should pay more attention and strengthen infection control. Department of surgery and ICU were the focus of infection prevention and control.