【Abstract】Objective To evaluate the expression of hTERT and α-Tubulin in cervical squamous cell carcinoma and its clinical significance. Methods The expression of hTERT and α-Tubulin in 20 specimen of normal cervical tissues, 40 specimen of CIN and 72 specimen of cervical squamous cell carcinoma were detected by immunohistochemical study, and their relations with clinical pathological features were analyzed. Results The positive expression rates of hTERT and α-Tubulin in cervical squamous cell carcinoma were 84.72% and 79.17%, respectively, which were higher than those in normal cervical tissues and CIN (P<0.05). hTERT expression were of no correlation with other clinical pathological parameters (P>0.05). α-Tubulin expression were significant correlation with clinical staging of tumor and lymph node metastasis (P＜0.05), while they were of no correlation with other clinical pathological parameters (P>0.05). The expression of hTERT was in positive correlation to that of α-Tubulin (r=0.353, P=0.002). Conclusion The expression of hTERT and α-Tubulin protein plays an important role in the development and progression of cervical squamous cell carcinoma. It is of great clinical significance for the early diagnosis of cervical squamous cell carcinoma.