【Abstract】Objective To study the clinical effect of internal mucosal resection and repair of fistula for the treatment of high anal fistula. Methods From January 2015 to July 2016, 86 patients with high anal fistula were selected in our hospital. The patients were divided into observation group and control group. Observation group were taken the mouth of the mucosal flap to repair the auxiliary fistula partial stripping. The control group was treated with the traditional way to complete the anal fistula incision hanging thread.The clinical efficacy, anorectal pressure, anal function, postoperative pain and sequelae were observed. Results After operation, there was no significant difference in the total effective rate between the observation group and the control group (P＞0.05). The anal resting pressure, rectal resting pressure and anal systolic pressure in the observation group were significantly higher than that in the control group (P＜005). The subjective score of anal function and anal advice score in the observation group were significantly lower than that in the control group (P＜0.05). The VAS score of the observation group was significantly lower than that of the control group (P＜0.05). The incidence of anal deformation, anal incontinence and anal dampness complication in the observation group were significantly lower than the control group (P＜0.05). Conclusion The internal mucosal resection and repair of fistula for the treatment of high anal fistula patients bring the patient less the pain, the low incidence of complications and good clinical curative effect.