【Abstract】 Objective To investigate the expression of human tumor susceptibility gene 101 (TSG101) in human malignant glioma and its relationship with the proliferation and metastasis of human glioma cells. Methods The clinical data and tumor tissues in 34 malignant glioma patients were collected from July 2015 to October 2016 in the department of neurosurgery in our hospital. The normal brain tissues in the 19 cerebral trauma patients were collected as control. PCR Realtime technology was used to detect the expression level of TSG101 in clinical samples. Spearman correlation analysis was used to detect the correlation between the expression level of TSG101 and the clinical pathological parameters of patients. Western Bolt was used to detect the expression level of TSG101 in the different human glioma cell line. The highest expression of TSG101 was selected to construct interference TSG101 expression cell lines,. Western Bolt and Realtime PCR was used to verify. The MTT method was used to detect the cell proliferation ability, and Transwell invasion assay was used to detect the ability of cell invasion. Results The results of Realtime PCR showed the expression of TSG101 in malignant glioma tissues was significantly lower than that in normal brain tissue (P<0.05). Spearman correlation analysis showed that the correlation between expression level of TSG101 and the gender, age and body weight was not significantly, and the grade of patients with glioma cells was negatively correlated. The results of Western Bolt showed that TSG101 in U251 cells was highly expressed compared with that in other cells. After successfully interfered with the expression of TSG101 in U251cells in vitro, the proliferation ability of U251 siTSG101 cells was significantly increased in 48h and 72h (P<0.05) after the expression of TSG101 in vitro. Transwell invasion assay showed significant enhancement of the cell invasion ability of interfering TSG101 expression (P<0.05). Conclusion The expression of TSG101 in malignant glioma tissue is low. Its expression is negatively correlated with the degree of malignancy of gliomas. It plays an inhibitory role in the proliferation and invasion of glioma.