【Abstract】 Objective To investigate the clinical application of chromosomal microarray analysis (CMA) in prenatal diagnosis for fetal echogenic intracardiac foci (EICF) detected by ultrasound. Methods From September 2014 to October 2016, amniotic fluid samples from 183 pregnancies with EICF were detected by CMA with Affymetrix CytoScan 750K arraysin our department. The results were analyzed by CHAS v31 software. Results Among the 183 cases, abnormalities were detected by CMA in 9 cases (492%). Of these 9 abnormal results, 3 were common aneuploidies (1 trisomy 21, 1 Klinefelter syndrome and 1 XYY) (164%), and 6 were with pathogenic copy number variations (pCNV) (including 1 16p1311 recurrent microdeletion) (328%). Conclusion Comparing with the traditional karyotyping and other rapid aneuploidy testings, CMA has its advantage over detecting extra clinically significant microdeletions/ microduplications, which significantly increases the detection rate of abnormal fetus with EICF and effectively reduce the occurrence of birth defects.