【Abstract】 Objective To investigate the expressions of ING4 and VEGF in prostate cancer and relationship of ING4 and VEGF in the development of tumor. Methods Expression of ING4 and VEGF in 28 cases of prostate cancer and 32 cases of benign prostatic hyperplasia were detected by EliVisionTM plus immunohistochemical stain. The immunoassay results were analyzed in relation to the tumor pathological grade, clinical stage and the different PSA. Results The positive expression rate of ING4 gene in prostate cancer tissues was significantly lower than that of benign prostatic hyperplasia, and decreased with the increase of tumor pathological grade, clinical stage and PSA. There was no difference in the positive expression rate of VEGF in prostate cancer and benign prostatic hyperplasia (P＞005). But the strong positive expression rate in prostate cancer was significantly higher than that in benign prostatic hyperplasia, and increased with the pathological grade, clinical stage and PSA. The expression of ING4 was negatively correlated with VEGF in prostate cancer, which was statistically significant(P＜005).Conclusion Abnormal expressions of ING4 is one of the significant factor of the increasing of the degree of the occurrence and progression of prostate cancer, and possibly play a role in tumor promotion by promoting tumor angiogenesis. ING4 may be a biological index for diagnosis and treatment of prostate cancer.