Abstract:【Abstract】 Objective To study the effect of high glucose on the expression of transient receptor potential cation channel protein 6 in immortalized mouse podocyte.Method The mouse podocyte were divided into hypertonic control group (mannitol 30mmol/l+ glucose 5mmol/l), normal control group (5mmol/L glucose), experimental group (glucose 15mmol/L, glucose 25mmol/L and glucose 35mmol/L) and high glucose (25mmol/l), respectively. The morphological changes of the cells were observed under the inverted microscope at 0h, 12h, 24h and 48h on podocyte. Immunocytochemistry was used to detect the distribution of TRPC6 expression in the podocytes. Western blotting and Reverse transcription polymerase chain reaction were used to detect the expression of TRPC6 protein in podocyte.Results With the increase of glucose concentration, the foot cell bodies became small and the foot processes were significantly decreased or disappeared. Immunocytochemistry showed TRPC6 expressed mainly in the podocyte cell membrane. The foot process distribution was obvious, WB and RTPCR detected in TRPC6 protein and mRNA expression increased gradually. Compared with prolonging of stimulation time, the podocyte protein TRPC6 and mRNA expression of 0h group was significantly increased (P＜005). The peak of podocyte protein TRPC6 and mRNA expression was at 48h and time dependent.Conclusion High glucose can lead to podocyte morphological change and cell damage, with an increase in the concentration of glucose increased and time, which can lead to podocyte TRPC6 expression increased with a dose dependent and time dependent.
