Objective To explore the standard method of perioperative prophylactic antibiotics applied to clean surgery so as to the reasonable use of antibiotics.Methods We compared the medical records of 100 patients collected before the intervention(from June of 2012 to Aug of 2012)with those of 100 patients after the intervention(from June 2013 to Aug 2013).Results Of the total 100 cases collected before intervention,all(100%)received antibiotics,with 78cases(78.0%)receiving antibiotics 0.5~2hours before operation,14cases(14.00%)2~24hours before operation,and 8cases(8.00%)after operation.Postoperatively,the rational ratio of course of antibiotic treatment was 8cases(8.00%),the mean cost of antibiotics was 934.7Yuan,and the mean hospitalization cost was 7083.1Yuan.The average hospitalization time was 295.2hours.Of the 100 cases after intervention,16cases(16.00%)were given antibiotics,with 100cases(100.0%)receiving antibiotics 0.5~2hours before operation and 0cases(0.00%)after operation.Postoperatively,the rational ratio of course of antibiotic treatment was 100cases(100.00%).The mean cost of antibiotics was 2.0Yuan,and the mean hospitalization cost was 4671.4Yuan.The average hospitalization time was 162.2hours The infection rate showed no statistical difference before and after intervention.Conclusion The intervention of pharmacist to the prophylactic antibiotic application can apparently improve the level of rational drug use during peroperative period.