Objective This study aims were to evaluate whether the single nucleotide polymorphisms(SNP)of the ANRIL gene were associated with increased risk of atherosclerosis stroke(ATS)in Chinese Han population in Northeast Sichuan.Methods 200 patients with ATS of Chinese Han population in Nanchong region(Northeast Sichuan)included as case group and 205 healthy people as control group.The genotyping of 4SNPs in ANRIL gene were tested and analyzed,as well as the association of SNPs with ATS were compared.Subgroup analyses were also carried out to determine whether the effect of the ANRIL polymorphism is specific to age and gender in participants.Results Dyslipidemia and overweight were significantly associated with ATS(P <0.05).GG genotype of rs1537378 and AA genotype of rs7865618 were significantly associated with ATS in male subgroup(P<0.05),while GG genotype of rs1537378 was significantly associated with ATS in ≥45year’s old subgroup(P<0.05),which could increase ATS susceptibility.Conclusions Dyslipidemia and overweight are risk factor for ATS.SNPs of ANRIL genes are associated with ATS in Han nationality of Chinese population.