Objective To explore the anti-cancer property of piperlongumine(PL)in gastric cancer BGC-823 cells and its underlying molecular mechanism.Methods Three specific concentrations(0μM,2μM and 20μM)of PL were included in current study.At different time points(0h,24 hand 48h),the cell viabilities of gastric cancer BGC-823 cells were calculated by MTT assay.Meanwhile,the mRNA expression of p53 was measured by qPCR.Then,Western blot was performed to analyze the protein expression of p53 and the phosphorylation of PI3 Kand Akt.Results Gastric cancer BGC-823 cells viabilities were dosage-dependently and time-dependently inhibited by PL.Meanwhile,after 48 hours of administrations,our data figured out that p53 expression was dosage-dependently up-regulated and the phosphorylation of PI3 K and Akt were dosage-dependently reduced by PL in gastric cancer BGC-823 cells.Conclusion The anti-tumor property of piperlongumine(PL)very likely results from up-regulation of p53 through the inactivation of PI3K/Akt signal pathway in gastric cancer BGC-823 cells,provides the promising of the clinic use of piperlongumine in the treatment of gastric cancer and other malignant tumors in future. 更多