Objective To analyze the effect of suture hemostasis in laparoscopic oophorocystectomy on the ovarian reserve function， sex hormone levels and stromal blood flow.Methods 106 patients who underwent laparoscopic ovarian cyst removal in this hospital from March 2019 to April 2020 were selected and divided into control group (electrocautery) 51 cases and observation group (sutures hemostasis) according to the different methods of hemostasis during the operation. The ovarian reserve function ［Antral follicle (AFC)， peak systolic blood flow velocity (PSV) of the affected ovarian artery］， sex hormone levels［Follicle stimulating hormone (FSH)， Luteinizing hormone (LH)， Estradiol (E2)］， stromal blood flow ［pulsatility index(PI) of stroma of ovary， resistance index (RI)， diastolic minimum velocity (EDV)， the ratio of systolic blood flow velocity to end-diastolic blood flow velocity (S/D)］and stress indicators ［cortisol (Cor)， adrenocorticotropic hormone (ACTH)， and norepinephrine (NE)］ before and after surgery were compared between the two groups.Results There were no differences in ovarian reserve， sex hormone levels， stromal blood flow， and stress indicators between the two groups before surgery (P>0.05).After operation， the AFC of the control group decreased， but there was no significant difference in the observation group compared with that before the operation (P>0.05). The PSV of the two groups decreased. The AFC and PSV of the observation group were higher than those of the control group (P<0.05). The FSH and LH levels of the observation group were significantly lower than those of the control group， and the E2 level was higher than that of the control group (P<0.05). After surgery， PI， RI， EDV decreased， and S/D increased， while PI， RI， S/D of the observation group were lower than those of the control group， and the EDV value was higher than that of the control group (P<0.05). The levels of Cor， ACTH and NE after surgery in the two groups were higher than those before surgery， and the levels of Cor， ACTH and NE in the observation group were lower than those in the control group (P<0.05).Conclusion Suture hemostasis during laparoscopic ovarian cystectomy can protect the patient's ovarian reserve function， stabilize the blood flow of the ovarian stroma， and alleviate the stress response， which is an ideal hemostasis method.