To explore the correlation between exhaled nitric oxide (FeNO) and different levels of eosinophils (EOS) in patients with stable chronic obstructive pulmonary disease (COPD) and its clinical application value. Methods A retrospective analysis was conducted on 187 stable COPD patients admitted to Peking University Shenzhen Hospital. They were divided into groups A and B based on whether the EOS percentage was greater than 2% (group A with EOS%＜2% and group B with EOS%≥2%). Basic information concluded(age, gender, body mass index (BMI), smoking status, etc.), FeNO value, blood EOS value, neutrophil count (NES), lung function parameters (FEV1/FVC). The COPD assessment test (CAT) scores were collected and statistically analyzed. Results The blood EOS% of Group B was higher than that of Group A, with a statistically significant difference (P＜0.001). The NES value of Group A was higher than that of Group B, with a statistically significant difference (P＜0.001); There was no statistically significant difference in FeNO (P=0.457), FEV1/FVC (P=0.166), and CAT score (P=0.114) between the two groups. The FeNO value of Group A patients was not correlated with blood EOS% (P=0.870), FEV1/FVC (P=0.255), CAT score (P=0.962), and NES (P=0.611). The FeNO value of Group B patients was positively correlated with blood EOS% (r=0.343,P=0.001), but not with FEV1/FVC (P=0.903), CAT score (P=0.617), and NES (P=0.836). Conclusion When the blood EOS level is low (EOS%＜2%), there is no significant correlation between the FeNO value and blood EOS% in stable COPD patients. When the blood EOS level is high (EOS%≥ 2%), the FeNO value of stable COPD patients is positively correlated with the blood EOS%, which can reflect the airway EOS inflammation of patients to a certain extent and has certain application value in the diagnosis and treatment of COPD