To study the heme ferritin feline-exporting protein feline leukemia virus subgroup C receptor (FLVCR) and the human breast cancer resistance protein (BCRP) after cerebral ischemia/reperfusion (I/R) in rats changes in expression and the regulatory effect of miR-137 on its expression.Methods60 SD rats were randomly divided into sham operation group (SO) and model group (MCAO/R), miR-137 mimic group (Mimic), miR-137 mimic control group (Mimic control), miR-137 inhibitor group (Inhibitor), miR-137 inhibitor control group (Inhibitor control).Rat model of middle cerebral artery occlusion-reperfusion (MCAO/R) was established by threading method, rats in each group were sampled 12h and 24h after reperfusion (n=10).The mRNA expression of FLVCR, BCRP and miR-137 in rat brain tissue was measured by qRT-PCR, and immunohistochemical was used to observe and evaluate the expression levels of FLVCR, BCRP proteins.Results Rat neurological deficit score: MCAO/R group score was significantly increased (P＜0.01); The score of the Mimic group decreased significantly (p＜0.01); The score of the Inhibitor group was significantly elevated (P＜0.05).miR-137 mRNA expression level: the mRNA level of the MCAO/R group was significantly reduced(P＜0.05); compared with the Mimic control group at the same time, the mRNA level in the Mimic group was significantly increased (P＜0.01) and 24h the elevation was more pronounced (P＜0.05); compared with the Inhibitor control group at the same time, the mRNA level of the Inhibitor group was significantly reduced (P＜0.05), and the 24h decrease was more significant (P＜0.05).FLVCR mRNA and protein expression levels: compared with the SO group, the expression level of the MCAO/R group was significantly reduced (P＜0.05)；compared with the Mimic control group, the expression level of the Mimic group was significantly increased at 12h (P＜0.05), 24h maximum (P＜0.05); compared with the Inhibitor control group, the expression level of the Inhibitor group decreased (P＜0.01) at 24h the decrease is more pronounced (P＜0.05).BCRP mRNA and protein expression levels: compared with the SO group, the expression level of MCAO/R group was significantly reduced (P＜0.05);compared with the Mimic control group, the expression level of the Mimic group was significantly increased at 12h (P＜0.01), at 24 hours highest (P＜0.05); compared with the Inhibitor control group, the expression level of the Inhibitor group was significantly reduced (P＜0.01). Conclusion miR-137 overexpression may promote the expression of FLVCR and BCRP for protection cerebral ischemia and reperfusion injury in rats