Abstract:To investigate the effect of SGLT2 inhibitor on lncRNA expression profiles in PDK1 knockout mice with heart failure. Methods PDK1 knockout mice were divided into control group and dapagliflozin(DAPA) treatment group. Arraystar mouse lncRNA microarray was used to analyze lncRNA expression profile by sequencing and screening. The real-time quantitative PCR was used to verify differentially expressed LncRNA. The lncRNA- mRNA co-expression network. Results KO+DAPAgroup significantly prolonged the survival of heart failure mice. There were 2653 lncRNAs in the two groups whose expression levels changed >1.5 times; bioinformatics analysis showed that lncRNAs were involved in energy metabolism. The differential expression was greater than 3 times and the above-mentioned 7 LncRNAs homologous to human and mouse construct a lncRNA-mRNA co-expression network, suggesting that uc.347 and uc.321 are associated with 141 mRNAs and 108 mRNAs, respectively. The overexpression of uc.347 in cardiomyocytes promotes the production of ATP energy. Conclusion This study revealed that dapagliflozin affects the lncRNA expression profile of PDK1 knockout mice with heart failure, and the differentially expressed lncRNA may be involved in the process of energy metabolism, thereby improving the prognosis of heart failure