To investigate the influence of vascular endothelial cell-derived exosomal miR-214 on H2O2-induced vascular endothelial cell injury. Methods Human umbilical vein endothelial cells (HUVEC) were cultured in vitro, and transfected with miR-214 inhibitor (in-miR-214) and its negative control (in-miR-NC), and exosomes were isolated and identified, and miR-214 expression was detected. HUVECs were cultured again and separated into normal control (Control) group, oxidative damage (H2O2) group, blank exosome + oxidative damage (exo NC+H2O2) group, in-miR-NC exosome + oxidative damage (exo in-miR-NC+H2O2) group and in-miR-214 exosome+oxidative damage (exo in-miR-214+H2O2) group. QRT-PCR was performed to detect the expression level of miR-214 in cells. MTT and flow cytometry were performed to detect cell proliferation and apoptosis. The scratch healing assay was performed to detect cell migration. The Western blot was performed to detect cell proliferation, apoptosis, migration-related protein levels. Results Exosomes were successfully isolated from HUVEC cells, and transfection of in-miR-214 could inhibit the expression of miR-214 in exosomes (P＜0.05). After H2O2 induction, the proliferation activity and scratch healing rate of HUVEC cells decreased, and the apoptosis rate increased. The expression level of miR-214 in the cells increased, the protein levels of Cyclin D1, PCNA, Bcl2, MMP2 and MMP9 decreased, and the protein level of Bax increased (P＜0.05). Adding HUVEC exosomes and exosome-derived in-miR-214 could partially attenuate H2O2 damage to HUVEC cells, and the effect of the latter was significantly better than that of the former (P＜0.05)