To analyze the role of serum amylase, albumin combined with lactate dehydrogenase in the clinical diagnosis and evaluation of acute pancreatitis. Methods The clinical data of 123 patients with acute pancreatitis treated in Beijing Shijitan Hospital Affiliated Capital Medical University Hospital from February 2018 to October 2021 were retrospectively analyzed, and they were set as the study group, at the same time, 87 patients who underwent physical examination during the same period were selected as healthy group. The levels of amylase, albumin, and lactate dehydrogenase in the study group and the control group, and inpatients with different disease levels were compared, the risk factors that affected the occurrence of acute pancreatitis were analyzed, and the clinical diagnostic value of serum amylase and albumin combined with lactate dehydrogenase for acute pancreatitis was analyzed. Results The levels of amylase and lactate dehydrogenase in the study group were higher than those in the healthy group, and the albumin level was lower than that in the healthy group(P＜0.05). There was no statistically significant difference in amylase level between the two groups (P＞0.05), the level of lactate dehydrogenase in the mild group was lower than that in the severe group, and the albumin level was lower than that in the severe group (P＜0.05). The results of multivariate logistic regression analysis showed that binge eating, alcohol abuse, amylase,albumin, and lactate dehydrogenase were independent risk factors that affected the occurrence of acute pancreatitis (P＜0.05). ROC curve showed: the AUC of amylase, albumin, lactate dehydrogenase tested separately and the three combined detection were 0.793, 0.701, 0.725, 0.896, respectively, of which the AUC value of the combined detection of the three was the largest. Conclusion The detection of serum amylase and albumin combined with lactate dehydrogenase can be used in the clinical diagnosis of acute pancreatitis, and provide effective data for accurately assessing the patient's condition