To explore the risk factors of non-suicidal self-injury(NSSI) behavior in adolescents with depression.Methods Collect the general clinical data of 291 patients with depression, biochemical indicators include three factors of anemia, blood lipid eight, glycosylated hemoglobin, hypersensitive C-reactive protein (hs-CRP), uric acid level, etc. According to the occurrence of NSSI, the patients were divided into two groups: group with NSSI (163 cases) and group without NSSI (128 cases). Chi-square test was used to analyze the enumeration data, T test was used to analyze the measurement data, and Logistic regression analysis was used to analyze the relevant factors.Results Adolescent depression patients with traumatic experience, inducement, high ferritin level and low HDL-C level were more likely to have NSSI.Conclusion Social-psychological factors are important influencing factors for NSSI in adolescent patients 〖JP2〗with depression. Ferritin and HDL-C are expected to be potential predictors for NSSI in adolescent patients with depression