To observe the intervention effect of Ziyin Liangxue Decoction on inflammatory factors and related podocyte marker proteins in chronic nephritis patients with Qi-yin deficiency and blood stasis syndrome, laying a good foundation for further study of its therapeutic mechanism and pathway.Methods Fifty-three patients diagnosed with chronic glomerulonephritis in the Department of Nephrology, Shanghai Hospital of Traditional Chinese Medicine from January 2019 to September 2021 were selected as the study objects, and the patients were randomly divided into a treatment group (28 cases) and a control group (25 cases). All patients were given basic treatment, control group was additionally given irbesartan tablets 150 mg orally, and treatment group was additionally given nourishing Yin and cooling blood decoction. The TCM syndrome integral and 24 h urine protein quantitative UPro (24 h), urinary albumin/uric creatinine and kidney function, tumor necrosis factor-alpha (TNF-alpha) protein, sugar, calyx (PCX) protein (Nephrin), kidney disease, glomerular podocyte fissure membrane protein (Podocin) were observed.Results 24 h-UPro, urine albumin/uric creatinine of the two groups before treatment were significantly different from that of 24 h-UPro, urine albumin/uric creatinine of the two groups after treatment (P＜0.05). 24 h-UPro, urine albumin/uric creatinine before and after treatment of treatment group were not significantly different from that of control group (P＞0.05). TNF-α before and after treatment of the two groups were significantly different (P＜0.05). PCX of treatment group after treatment was different from that of before treatment (P＜0.05). PCX of control group after treatment was not different from that of before treatment (P＞0.05). PCX after treatment of treatment group was different from that of control group (P＜0.05). Urinary Nephrin and Podocin of the two group before and after treatment were significantly different (P＜0.05). TCM symptom integral of the two group before and after treatment were significantly different (P＜0.05). In the course of clinical observation, no adverse events occurred in the treatment group and the control group. Conclusion Compared with the control group, the treatment group taking Ziyin Liangxue Decoction can relieve the related symptoms of chronic nephritis patients, improve the immune inflammation damage and glomerular podocyte damage, and reduce proteinuria and delay disease progression