To evaluate the growth and healing of periodontal tissue after autologous heterotopic transplantation. Methods A 1-year-old beagle dog was used in the experiment. The left mandibular third incisor was extracted and frozen in 10% dimethyl sulfoxide preservation solution -80 ℃ for 2 weeks. After programmed resuscitation and thawing, it was transplanted into the same tooth position of the right mandibular. The left mandibular second incisor was immediately transplanted into the same tooth position of the right mandibular as the control. It was fed for 3 months. The periodontal healing was evaluated by clinical observation, X-ray examination, micro CT and histological staining. Results WOne month after operation, the gingival margin of frozen transplanted teeth and immediate transplanted teeth was slightly red and swollen BOP (probing bleeding) + loosening Ⅰ°. X-ray film showed low-density transmission image around the frozen tooth root tip, without internal and external root absorption image. 3 months after operation, the gums of both teeth were red and swollen, BOP +, loosening Ⅰ-Ⅱ°, probing and mild periodontal pocket. X-ray film showed concave absorption in varying degrees on the surface of both teeth root, obvious absorption of cementum near the middle root tip of frozen teeth, and no internal root absorption image. Micro CT The images showed that 3 months after immediate tooth transplantation, the root surface was slightly rough, there was slight superficial absorption in some parts, and there was no penetrating shadow in the apical area. A large range of absorption occurred 3 months after cryotransplantation, affecting the dentin, accompanied by apical alveolar bone absorption. Histological HE staining sections showed that the root surface of frozen teeth was absorbed, and the reattachment of cervical junction epithelium was loose. The root resorption of the control teeth was lighter, and the reattachment of the binding epithelium was more dense. Conclusion With theselection of appropriate cryoprotectants and freezing methods, the periodontal prognosis of frozen transplanted teeth can be expected, but more research is still needed to prevent root resorption.