【Abstract】 Objective To explore the effect of jujuboside A on the proliferation and differentiation of mouse MC3T3-E1 osteoblasts，and provide an experimental basis for clinical treatment of fractures and promotion of fracture healing. MethodsJujuboside A was divided into 4 concentrations (5，10，20 and 40 mg/L). Cell proliferation was detected by CCK-8，cell apoptosis was detected by flow cytometry，the cell mineralized nodule formation was detected by Von Kossa staining，the content of ALP and BGP in cells was determined by Elisa，expression levels of Runx-2 and BGP in MC3T3-E1 cells were detected by RT-qPCR，expression levels of TGF-β1，Col I and BMP-2 in MC3T3-E1 cells were detected by Western Blot. ResultsJujuboside A can promote the proliferation of MC3T3-E1 cells，reduce the rate of cell apoptosis，increase the content of ALP and BGP in the cells，increase the expression levels of Runx-2 and BGP genes and the protein expression levels of TGF-β1，Col I and BMP-2，and increase the number of mineralized nodules in the cells (P<0.05). ConclusionJujuboside A can promote the proliferation of MC3T3-E1 osteoblasts，reduce cell apoptosis，improve the degree of cell differentiation and osteogenic efficiency，and its mechanism may be exerted by regulating the TGF/BMP pathway.