β-谷甾醇缓解LPS诱导的急性肺损伤大鼠炎症反应和纤维化
DOI:
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

河北省医学科学研究重点课题计划


β-sitosterol alleviated inflammation response and fibrosis in rats with LPS-induced acute lung injury
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    【摘要】 目的 探讨β-谷甾醇(β-sitosterol)对脂多糖(LPS)诱导的急性肺损伤大鼠炎症反应和纤维化的改善作用。方法采用LPS法构建急性肺损伤大鼠模型,将60只SD大鼠随机分为健康对照组、模型组、模型+β-sitosterol组(5、10、20 mg/kg),每组12只。采用TUNEL染色检测肺组织细胞凋亡,ELISA检测外周血炎性因子白细胞介素(IL-1β、IL-4、IL-10)、肿瘤坏死因子α(TNF-α)及诱导型一氧化氮合酶(iNOS)的含量,肺天狼星红染色观察肺组织病理变化,western blotting和RT-PCR检测肺组织中增殖标记物Ki67、半胱天冬酶3(Cas-3)、iNOS、IL-4、α-平滑肌蛋白(α-SMA)、转化生长因子β(TGF-β)及纤维连接蛋白(FN)的表达。结果与健康对照组比较,模型组大鼠肺组织出现细胞凋亡、炎性细胞浸润和纤维化,肺组织中Ki67蛋白的相对表达量下调,外周血IL-1β、TNF-α、iNOS、IL-4、IL-10、肺组织中切割后Cas-3(cleaved Cas-3)/Cas-3水平、iNOS、IL-4 mRNA及蛋白、α-SMA、TGF-β及FN蛋白的相对表达量上调(均P<0.05);与模型组比较,10、20 mg/kg β-sitosterol组外周血IL-4、IL-10、肺组织中Ki67蛋白的相对表达量、IL-4 mRNA及蛋白的表达上调(均P<0.05),外周血IL-1β、TNF-α、iNOS、肺组织中cleaved Cas-3/Cas-3水平、iNOS mRNA及蛋白、α-SMA、TGF-β及FN蛋白的相对表达量下调(均P<0.05)。结论β-谷甾醇可能通过抑制肺组织细胞凋亡、降低炎症反应及减轻纤维化,改善LPS诱导的急性肺损伤。

    Abstract:

    【Abstract】 Objective To explore the improvement effects of β-sitosterol on inflammatory response and fibrosis in rats with acute lung injury induced by lipopolysaccharide (LPS). MethodsThe rat models of acute lung injury were constructed by LPS method. A total of 60 SD rats were randomly divided into healthy control group,model group and model+β-sitosterol group (5,10,20 mg/kg). The apoptosis of lung tissues was detected by TUNEL staining. The contents of peripheral blood inflammatory factors [interleukin (IL-1β,IL-4,IL-10),tumor necrosis factor α (TNF-α),inducible nitric oxide synthase (iNOS)]were detected by ELISA. The pathological changes of lung tissues were observed by Sirius red staining. The expression of proliferation marker Ki67,caspase 3 (Cas-3),iNOS,IL-4,α-smooth muscle actin (α-SMA),transforming growth factor β (TGF-β) and fibronectin (FN) was detected by western blotting and RT-PCR. ResultsCompared with healthy control group,there was apoptosis of lung tissues,inflammatory cells infiltration and fibrosis in model group,relative expression of Ki67 protein was down-regulated,levels of peripheral blood IL-1β,TNF-α,iNOS,IL-4 and IL-10,and relative expression levels of cleaved Cas3/Cas3,iNOS,IL-4 mRNA and protein,α-SMA,TGF-β and FN protein were up-regulated (P<0.05). Compared with model group,levels of peripheral blood IL-4 and IL-10,relative expression level of Ki67 protein in lung tissues,expression of IL-4 mRNA and protein were up-regulated in 10 and 20 mg/kg β-sitosterol groups (P<0.05),while levels of peripheral blood IL-1β,TNF-α and iNOS,cleaved Cas3/Cas3 level in lung tissues,and relative expression levels of iNOS mRNA and protein,α-SMA,TGF-β and FN protein were down-regulated (P<0.05). Conclusionβ-sitosterol may reduce inflammation response and fibrosis by inhibiting apoptosis in lung tissues,thus improving LPS-induced acute lung injury.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2022-06-20
您是第位访问者
版权所有:《西部医学》编辑部     蜀ICP备18038379号-4
地址:四川省成都市武侯区小天竺街75号财富国际18F-1号    邮政编码:610041
电话:028-85570072/85588403    E-mail:xbyxqk@163.com
技术支持:北京勤云科技发展有限公司