【Abstract】Objective To explore the expression of microRNA (miR)-146b-5p and miR-134 in liver cancer tissues and their clinical pathological significance. Methods The cancer tissues specimens from the 73 patients with primary liver cancer were enrolled. The corresponding para-cancerous normal tissue specimens were enrolled as controls. The expression levels of miR-146b-5p and miR-134 in cancer tissues and para-cancerous normal tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR). The correlation between their expression levels and clinicopathological features was analyzed. Results The relative expression levels of miR-146b-5p and miR-134 in liver cancer tissues were lower than those in para-cancerous tissues (P＜0.05). The down-regulated expression of miR-146b-5p was related to tumor size (longer than 5cm), non-venous infiltration and TNM staging (stage Ⅲ/Ⅳ) (P＜0.05). The down-regulated expression of miR-134 was related to tumor size (longer than 5cm), cirrhosis, TNM staging (stage Ⅲ/Ⅳ) and non-tumor capsule (P＜0.05). Conclusion In liver cancer tissues, the expression levels of miR-146b-5p and miR-134 are down-regulated, which may be involved in the occurrence and development of liver cancer, and are closely related to clinical malignant phenotypes of liver cancer.