miR-215-5p在甲状腺滤泡癌中的表达及与血清TSH的相关性
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河北省医学科研研究课题(20190232)


Expression of miR-215-5p and its correlation with serum TSH in thyroid follicular carcinoma
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    摘要:

    目的 观察甲状腺滤泡癌组织中微小RNA-215-5p(miR2155p)的表达特征,并分析miR-215-5p与血清中促甲状腺激素(TSH)的关系。方法 选取2014年3月~2017年4月河北省人民医院和河北以岭医院55例甲状腺滤泡癌术后组织为观察组,留取术前的血清标本,另选取55例甲状腺滤泡性腺瘤组织为对照组。应用实时荧光定量PCR(qPCR)检测miR-215-5p的表达,免疫组化法检测甲状腺滤泡癌组织中Ki67的表达,电化学发光法检测甲状腺滤泡癌患者血清TSH的表达。结果 甲状腺滤泡癌中miR-215-5p的表达明显低于对照组,miR-215-5p 的表达在不同肿瘤最大径、Ki67增殖指数和TNM分期的分组中差异有统计学意义(均P<0.05)。miR-215-5p的表达与预后相关,甲状腺滤泡癌组织中miR-215-5p与血清TSH呈负相关。结论 miR-215-5p在甲状腺滤泡癌中的表达下降,与病变的临床特征相关,且与TSH成负相关。miR-215-5p 的表达与患者预后有关。

    Abstract:

    Objective To observe the expression of miR-215-5p (miR-215-5p) in thyroid follicular carcinoma and analyze the relationship between miR-215-5p and TSH of serum. Methods 55 cases of thyroid follicular carcinoma were selected as the observation group. The serum samples were collected before operation. 55 cases of thyroid follicular adenoma were selected as the control group. Expression of miR2155p was detected by real time fluorescent quantitative PCR(qPCR). Expression of Ki67 was detected by immunohistochemistry method in thyroid follicular carcinoma. Serum expression of TSH was detected by chemiluminescence in thyroid follicular carcinoma.Results Expression of miR2155p was significantly lower in thyroid follicular carcinoma than that in the control group. The expression of miR-215-5p was statistically significant in tumor maximum diameter, Ki67 proliferation index and TNM stage. The expression of miR2155p is related to prognosis. Negative correlation was found between miR2155p and TSH of serum in thyroid follicular carcinoma. Conclusion Expression of miR2155p is decreased, which is related to the clinical characteristics in thyroid follicular carcinoma. Negative correlation is found between miR-215-5p and TSH. The expression of miR-215-5p is related to prognosis.

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  • 在线发布日期: 2021-04-01
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