Objective To study the effects of microRNA-378a-3p (miR-378a-3p) on proliferation, invasion and migration of hemangioma endothelial cells (HemECs) and its possible mechanism.Methods HemECs cells were randomly divided into control group, miRNC group, miR-378a-3p group. HemECs cells were cultured routinely in the control group. HemECs in the miR0-378a-3p group were transfected with miR-378a-3p mimics by Lipofectamine RNA IMAX and miRNC group were transfected with negative control plasmid. The expression of miR-378a-3p were detected by quantitative polymerase chain reaction (qPCR). The cells proliferation were detected by cell counting ki8(CCK-8), and the cells invasion and migration were observed by Transwell assay. Targeting relationship between Mir 378a 3P and kinesin superfamily 2A (Kif2a) was verified by online database TargetScan prediction and double luciferase reporter gene. Results Compared withthe control group, the miR-378a-3p mimic could significantly upregulate the expression of miR-378a-3p in HemECs cells (P＜0.05). Up-regulation of the expression of miR-378a-3p significantly inhibited the proliferation of HemECs cells (P＜0.05), and decreased invasion and migration (P＜0.05). KIF2A was one of the downstream target genes of miR-378a-3p. Conclusion miR-378a-3p may inhibit the proliferation, invasion and migration of HemECs cells by targeting KIF2A.