【Abstract】 Objective To investigate the slice effects of huwe1 shRNA on huwe1 gene in neuronal stem L23 cells. Methods The neuronal stem L23 cells were divided control group (control group), control shRNA group (control nonsilencing shRNA group) and huwe1 sliced group (huwe1 shRNA group). L23 cells in control group were cultured in Serumfree Medium. Control nonsilencing and rat huwe1 shRNA vectors were obtained from open Brosystems. Lentivival packaging was done in TLAHEK293T cells. L23 cells were transduced with lectiviral stock. All the neural stem cells in different groups were collected, and the expression of huwe1 protein was detected by western blot technology. Results Compared those in control group and control nonsilencing shRNA group, the expression of huwe1 protein huwe1 shRNA group were significantly decreased. Conclusions Knockdown huwe1 by GIPZ shRNA lentiviral vector decreased the expression of huwe1 protein in L23 cells.