Abstract:【Abstract】Objective To establish a rat model of myocardial ischemia reperfusion injury (MIRI) and observe the protein and mRNA expression characteristics of inflammatory factors including peroxisome proliferator activated receptor ɑ (PPARɑ), cytochrome P4501A1 (Cyp4501A1), phosphatidylinositol 3kinase (PI3K) and receptor serine/threonine kinases (AKT) in the myocardium after biochanin A administration. Methods After adaptive feeding for two weeks, 60 SD rats were randomly divided into the sham operation group, model group, ramipril group, Biochanin A low, medium, and high dose group. Two weeks after gavage, all rats were used to construct Miri model by reversible ligation of anterior descending branch of left coronary artery. In the sham operation group, only thread was threaded without ligation. Immediately after operation, blood was collected to detect the myocardial enzyme spectrum and serum inflammatory factor content, and the expression characteristics of myocardial pathology, myocardial inflammatory index, PPAR, cyp4501a1, PI3K, Akt protein and mRNA were collected to detect. Results Myocardial histopathology showed that in the sham operation group, myocardial fibers were arranged in order, and there were fiber rupture and tissue necrosis in the model group. Compared with the model group, the myocardial enzymes CK MB and LDH, myocardial and serum inflammatory factors hs CRP, IL1 α, IL6, IL8 and TNFα in the model group were significantly higher (P<005), and the treatment groups were significantly higher (P<005). Compared with the model group, PPAR, cyp4501a1, PI3K, Akt protein and mRNA expression levels in the model group were significantly lower (P<005), while those in each treatment group were significantly higher (P<005). Conclusion Biochanin A has a significant inhibitory effect on the myocardial inflammatory factors in rats with MIRI, and the mechanism may be related to that it can regulate the proteins and RNA expression of PPARa, Cyp4501A1, PI3K, and AKT.
