Abstract:【Abstract】 Objective To explore clinical effect of masking therapy combined with gastrodin injection on sudden deafness (SD) with tinnitus. Methods 82 SD patients with tinnitus admitted to our hospital from April 2018 to September 2018 were randomly divided into observation group and control group by simple random number table method, 41 patients in each group. The control group was treated with gastrodin injection for 10 days, and the observation group was treated with masking therapy for 90 days. The curative effect of SD in the two groups was evaluated 10 days after treatment, and tinnitus in the two groups was observed 90 days after treatment. Before and after 10, 30 and 90 days of treatment, the tinnitus disability (THI) and tinnitus severity (TEQ) were assessed once respectively. Meanwhile, the serum levels of soluble vascular cell adhesion molecule (sVCAM 1) and high mobility group protein (HMGB1) were detected by enzyme-linked immunosorbent assay. Side effects related to treatment were recorded in both groups. Results The total effective rates of SD and tinnitus in the observation group were significantly higher than those in the control group (P<0.05). With the prolongation of treatment time, the scores of thi and TEQ in the two groups decreased gradually (P<0.05), and the scores of thi and TEQ in the observation group were lower than those in the control group at the same time point (P<0.05). With the prolongation of the treatment time, the serum levels of sVCAM 1 and HMGB1 in the two groups decreased gradually (P<0.05), and the serum levels of sVCAM 1 and HMGB1 in the observation group were significantly lower than those in the control group (P<0.05). No side effects were found in all patients. Conclusion The application of masking therapy combined with gastrodin injection in the treatment of SD with tinnitus is helpful to save the hearing of the patients with damaged frequency, improve the tinnitus symptoms, reduce the adverse effects of tinnitus on the patients, and improve the quality of life. The mechanism may be related to the decrease of the expression level of sVCAM 1 and HMGB1 in serum.