Abstract:【Abstract】Objective To investigate the effects of zingiberenone on proliferation, migration, invasion and apoptosis of HeLa cell line. Methods HeLa cell line of human cervical cancer was cultured and divided into blank group, 5μmol/L group, 10μmol/L group and 20μmol/L group. Cell proliferation, migration, invasion, apoptosis and relative expression of EMTrelated proteins were observed. Results At 24h, 48h and 72h, the cell proliferation rate in the 20 μmol/L group was lower than that in the 5 μmol/L group, the 10 μmol/L group and the blank group (P＜005). At 24h, 48h and 72h, the apoptosis rate of the 20 μmol/L group was higher than that of the 5 μmol/L group, the 10 μmol/L group and the blank group (P＜005). The cell migration and invasiveness of 20 μmol/L group were all lower than those of 5 μmol/L group, 10 μmol/L group and blank group (P＜005). The relative expression levels of Vimentin and Enhancer of zeste homolog 2 (EZH2) in human cells in the 20 μmol/L group were all lower than those in the 5 μmol/L group, the 10 μmol/L group, and the blank group. The relative expression levels of αcat and ecadherin were all higher than those of 5 μmol/L group, 10 μmol/L group and blank group (P＜005). Conclusion Zingiberenone can promote the apoptosis of HeLa cells and inhibit the proliferation and migration of HeLa cells. Invasiveness and capacity were concentration dependent.
