模拟正畸静压力刺激对牙周膜干细胞增殖及PI3K/AKT信号通路的作用
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乌鲁木齐市卫计委科研项目(201606)


The effect of simulating orthodontic static pressure stimulation on the proliferation of periodontal ligament stem cells and PI3K/AKT signaling pathway
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    摘要:

    【摘要】目的 探讨模拟正畸静压力刺激牙对周膜干细胞增殖及磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)信号通路的作用。方法 将体外培养至生长状态良好的牙周膜干细胞加压至100KPa处理1h、6h和12h后检测细胞的增殖情况,检测细胞凋亡相关蛋白及PI3K/AKT信号通路蛋白的表达。以置于加压舱不加压细胞为对照组(Con)。结果 分离纯化后的牙周膜干细胞具有多向分化能力,加压处理1h和6h后牙周膜干细胞的增殖受到明显的抑制;与对照组比较,含半胱氨酸的天冬氨酸蛋白水解酶(Caspase)6、Caspase9、PI3K及AKT的表达明显增强(均P<005);而处理12h后上述指标比较差异均无统计学意义(均P>005)。结论 模拟正畸静压力刺激对牙周膜干细胞增殖有明显的抑制作用,此过程可能与PI3K/AKT信号通路异常有关。

    Abstract:

    【Abstract】Objective To explore the effect of simulating orthodontic static pressure stimulation on the proliferation of periodontal ligament stem cells and phosphatidylinositol 3kinase (PI3K)/Protein kinase B (AKT) signaling pathway. Methods Cell proliferation was measured in vitro culture of periodontal ligament stem cells with good growth conditions under 100 KPa for 1 h, 6 h and 12 h. Apoptosis related proteins and PI3K/AKT signaling pathway proteins were detected. Results The separated and purified periodontal ligament stem cells had the ability of multidirectional differentiation. The proliferation of the stem cells in the posterior periodontal ligament of 1h and 6h was significantly inhibited by pressure treatment, and the expression of apoptotic protein cysteinyl aspartate specific proteinase (Caspase)6 and Caspase9 was significantly enhanced (P<001, P<005). The expression of PI3K/AKT pathway protein PI3K and AKT was significantly enhanced (P<001, P<005), and was significantly different from the control group (P<001, P<005), but there was no significant difference between the above indexes after treatment (P>005). Conclusion Simulated orthodontic static pressure stimulation significantly inhibits the proliferation of periodontal ligament stem cells, and this process may be related to abnormality of PI3K/AKT signaling pathway.

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  • 在线发布日期: 2019-11-13
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