【Abstract】Objective To investigate the infiltration pattern and the prognostic value of the tumorinfiltrating immune cell in cervical cancer. Methods Clinical information and gene expression profiles from TCGACESC (The Cancer Genome AltasCervical squamous cell carcinoma and endocervical adenocarcinoma) dataset were 〖JP2〗retrospectively collected.〖JP〗 CIBERSORT was used to determine the proportions of 22 immune cell types of 309 tumors. Cox regression model was performed to evaluate the association of tumorinfiltrating immune cells and immune checkpoint modulators with overall survival (OS).Results Of the 22 cell types, Macrophages (291%), gamma delta T cells (123%), activated CD4+ memory T cells (80%), follicular helper T cells (76%), plasma cells (67%) and inactivated mast cells (55%) were abundant in cervical cancer. Univariate analysis showed that high proportions of CD8+ T cells, activated CD4+ memory T cells and inactivated mast cells were associated with prolonged OS, while high proportions of inactivated CD4+ memory T cells and activated mast cells were associated with poor OS. Multivariate analysis showed that activated CD4+ memory T cells (P=0001, HR 0323, 95% CI 01800581) and activated mast cells (P=0003, HR 2433, 95% CI 13504387) were independent prognostic factors for survival of patients with cervical cancer. The mRNA expression levels of Immune checkpoint molecular (CD274, PDCD1, CTLA4, ICOS) were positively correlated with the infiltration level of CD4+ memory T cell, but negatively correlated with the infiltration level of activated mast cell. ICOS gene expression was an independent prognostic factor for survival of patients with cervical cancer (P=0019, HR 0687, 95% CI 05030939). Conclusion This study indicates that tumorinfiltrating immune cells play an important role in the prognosis of patients with cervical cancer, and reveal potential biomarkers and targets for immunotherapy of cervical cancer.