【Abstract】 Objective To explore the influence of irregular antibody detection on the safety of patients with clinical blood use and blood transfusion. Methods 186 patients were divided into observation group and control group according to the different time period of blood transfusion treatment, 93 cases each. The patients in the observation group were tested for irregular antibodies before blood transfusion(experimental method was microcolumn gel test), and preventive measures were taken. The control group did not carry out irregular antibody detection before blood transfusion treatment, and analyzed the occurrence of blood transfusion reaction in two groups of patients, and analyzed the irregular antibody detection and positive typing results of antibody screening in the observation group. Results The incidence of blood transfusion reaction in the observation group was 108 %, which was significantly lower than 1076 % in the control group(P>0.05). There was no significant difference between male and female in the results of irregular antibody detection(P >0.05). There was no statistically significant difference between patients with pregnancy history and those without pregnancy history(P > 0.05); The difference between patients with history of transfusion and patients with no history of transfusion was statistically significant(P<0.05). The positive types of irregular antibody screening in the observation group were mainly antiM and antiE, accounting for 3333 % and 22.23 %, respectively, and antiD, antis, antiK, and antic were relatively low. Conclusion Irregular antibody detection before whole blood transfusion and targeted preventive measures can effectively improve the safety of clinical blood use and reduce the incidence of transfusion reaction.