【Abstract】 Objective To investigate the effect of laparoscopic panhysterectomy with bilateral salpingectomy on the serum antimullerian hormone (AMH) and ovarian function of premenopausal women. Methods The clinical data of 168 patients with benign uterine diseases treated by laparoscopic panhysterectomy in Qionghai People's Hospital between January 2015 and January 2017 were retrospectively analyzed. Patients were divided into the observation group and the control group, each with 84 cases. The observation group and the control group were divided ≥45 years group and＜45 years group by age. The control group preserved bilateral fallopian canal, and the observation group underwent simultaneously bilateral fallopian tube resection. The surgical conditions, luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), AMH, ovarian arterial blood supply, perimenopausal symptoms, tubal lesions, and incidence rate of pelvic encapsulated fluid were compared. Results There was no significant difference in operation time, intraoperative blood loss, anus exhaust time and hospital stay between the two groups (P＞0.05). At postoperative 1mon, 3mon and 6mon, the LH and FSH levels were increased significantly, E2 and AMH levels were decreased significantly (P＜0.05), and no difference was found in the above levels between groups (P＞0.05). The level of AMH at 1, 3 and 6 months after operation in the observation group of＜45 years was significantly lower than that in the control group of＜45 years old (P＜0.05), and there was no significant difference in the level of AMH between the 1, 3 and 6 months after the operation in the observation group of 45 years old and that of the control group with the age of 45 years old (P＞0.05). There was no significant difference in the incidence of perimenopausal symptoms between the two groups at 1 month, 3 months, and 6 months after operation (P＞0.05). The incidence of fallopian tube lesions and pelvic encapsulated fluid in the observation group were significantly lower than those in the control group (000% vs 1905%, 357% vs 1667%, P＜0.05). Conclusion Compared with the panhysterectomy, laparoscopic panhysterectomy with bilateral salpingectomy can prevent the incidence of and pelvic encapsulated fluid, while the effect on the ovarian function, AMH, ovarian artery blood supply and perimenopausal symptoms are similar.