Abstract:【Abstract】 Objective To detect the expression of miR150 in cervical cancer cells C33A and investigate whether it promotes the proliferation and invasion of cervical cancer cells by regulating the target gene FOXO4.Methods The effects of miR150 on cell cycle and apoptosis, as well as the expression of cycleand apoptosisrelated genes, were determined using flow cytometry, TUNEL assay, qRTPCR, and Western blot, respectively. The direct target of miR150 was confirmed using 3' untranslated region (UTR) luciferase reporter assay.Results miR150 promotes cervical cancer cell C33A survival and growth, while the inhibition of miR150 suppresses these actions. miR150 also induced the cell cycle progressionfrom G1/G0 to S phase, resulting in an enhancement of growth. Several cell cycleand apoptosisrelated genes, CyclinD1, p27, BIM, and FASL were modulated by miR150.Moreover, miR150 directly reduced the expression of FOXO4, which regulates the expression of CyclinD1, p27, BIM, and FASL, by targeting its 3' UTR.Conclusion Our data demonstrate that elevated miR150 targets FOXO4 expression and therefore regulates multiple genes expression, resulting in cervical cancer cell C33A growth and survival.