【Abstract】 Objective To establish a stable and reproducible neonatal rat model of sepsisinduced myocardial damage and provide model animals for studying the pathogenesis and prevention of neonatal sepsis. Methods 54 10day age male SD neonatal rats were randomly divided into normal control group，sham group and cecal ligation and puncture (CLP) group. Colony growth was observed by blood culture. Serum cardiac troponin I (cTNI), antitumor necrosis factorα (TNFα) and interleukin1β (IL1β) concentration of myocardial tissue were detected by ELISA. Change of myocardial tissue was observed under light microscope after hematoxylin eosin staining. Results Negative blood culture results were found in the normal control group and sham group. A variety of pathogenic bacteria especially Escherichia coli were detected in CLP group. There was no significance between the concentration of cTNI, TNFα and IL1β in the normal control group and the sham group (P＞0.05). The concentration of cTNI, TNFα and IL1β were significantly higher in the CLP group than in the sham group (P＜0.05). According to results of hematoxylin eosin staining, there were no inflammatory changes in cardiac myocytes in normal control group and sham group, but inflammatory cell infiltration around cardiac myocytes was observed in CLP group. Conclusion For establishing the neonatal rat model of sepsisinduced myocardial damage, CLP is a preferable choice in our research.