Objective To observe the changes of pressure and intracranial pressure in epidural cavity of rabbits at different perfusion rates by perfusing the fluid into the epidural cavity. Methods 36 New Zealand rabbits were used in this experiment. All the rabbits were established intracranial pressure channels at the top of cranium and lower waist. The experimental animals were randomly divided into six groups with 6 animals in each group. The peristaltic pump was used to control the perfusion flow rate. Group A was without perfusion. Group B was with 0. 1 ml / min normal saline perfusion. Group C was with 0.5ml/min saline infusion. Group D was with 1.0ml/min saline infusion. Group E was with 0.5ml/min iodine infusion. Group F was with 1.0ml/min iodizol infusion group. The epidural pressure and intracranial pressure were recorded at the time of T0（0 min）, T1（2 min）, T2（ 4 min) T3 （8）min) T4 (5 min after perfusion) in each normal saline group. The ascending segments of contrast media in R1（1ml）, R2 （2ml）, R3（4ml）group were recorded. Results The epidural pressure and intracranial pressure increased with the increase of perfusion time, and then reached the plateau stage. With the increase of perfusion rate, the peak value of epidural pressure and intracranial pressure also increased, and there was statistical difference among the three groups (P＜0.05). There was no significant correlation between the rising height and velocity of epidural fluid under the same perfusion volume (P＞0.05). Conclusion The peak value of epidural pressure and intracranial pressure was positively correlated with perfusion velocity, but had no significant correlation with perfusion time (volume). There was no significant correlation between the rising height of perfusion fluid and the perfusion velocity, but a positive correlation with the perfusion time (volume)